Gene library synthesis by structure-based combinatorial protein engineering.

types of combinatorial libraries, such as random shuffling libraries and artificial alternative splicing libraries. The block shuffling and random point mutations can be introduced simultaneously by performing RMPCR under error-prone conditions. Most of the block sequences in the combinatorial libraries created by RM-PCR encode a long open reading frame and are suitable for protein selection experiments. Thus, RM-PCR promises to be a powerful approach in creating novel proteins, especially simulating early stages of protein evolution on a rapid timescale using an efficient and easily adaptable experimental protocol. [8] SCOPE 75